Physical State:Freeze-dried solid
Storage and Rehydration:Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration:Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.Expiration date:one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Buffer:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)Preservative:None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)Suggested Working Concentration or Dilution Range:
ELISA:- 1-2 μg / ml
Western Blot:- 0.01-0.1 μg / ml
Histo-/Cyto-Chemistry:- 1-2 μg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.