Superdex 200 Increase small-scale SEC columns
Superdex 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~10 000 to ~600 000.
Enhanced performance: improved resolution and runtime compared to predecessor Superdex 200.
Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
High flow rates: rigid beads give excellent pressure/flow properties.
“Increase”: Cytiva's size exclusion chromatography columns
Superdex 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex Increase columns deliver higher resolution purification in shorter run times.
| Parameter | Superdex 200 Increase 10/300 GL |
|---|---|
| Bed dimensions | 10 x 300 mm |
| Bed height | 300 to 310 mm |
| Bed support | Filter |
| Bed volume | ~ 24 mL |
| Chemical stability | Conditions where the resin may be operated without significant change in function: ? All commonly used aqueous buffers, pH 3 to 12 ? Urea, up to 8 M ? Ionic and nonionic detergents ? Guanidine hydrochloride, up to 6 M Conditions where the resin can be subjected to cleaning- or sanitization-in-place without significant change in function: ? Acetonitrile, up to 30% ? Sodium hydroxide, up to 1 M ? Ethanol, up to 70% ? Acetic acid, up to 1 M ? Isopropanol, up to 30% ? Hydrochloric acid, up to 0.1 M ? Trifluoroacetic acid, up to 10% ? Formic acid, up to 70% Avoid: ? Oxidizing agents ? Unfiltered solutions |
| Chemical stability (column hardware) | Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons |
| Column i.d. | 10 mm |
| Exclusion limit (Mr) (globular proteins) | ~ 1.3 × 106 |
| Fractionation range (Mr) (globular proteins) | 10 000 to 600 000 |
| Material (column hardware) | Borosilicate glass, polyetheretherketone (PEEK), polypropylene (PP), ethylene propylene diene monomer (EPDM), and perfluoro-rubber (PFR) |
| Material (bed support) | Polyethylene (PE) filter |
| Material (column tube) | Borosilicate glass |
| Matrix | Composite of cross-linked agarose and dextran |
| Operating flow rate, max. | 1.80 mL/min, water at room temperature; 0.90 mL/min, 20% ethanol or 10% glycerol at room temperature; 0.90 mL/min water at low temperature; 0.45 mL/min 20% ethanol or 10% glycerol at low temperature |
| Operating flow rate, recommended1 | 0.75 mL/min |
| Particle size, d50V2 | ~ 8.6 μm |
| Sample volume | 25 to 500 μL |
| Storage conditions | 4?C to 30?C, 20% ethanol |
| Theoretical plates | > 48 000 m-1 |
| Typical pressure drop over the packed bed3 | ~ 3 MPa |
| pH stability, cleaning-in-place (CIP) | 1 to 14 |
| pH stability, operational | 3 to 12 |
1Water at 20?C to 25?C.
2Median particle size of the cumulative volume distribution.
3At 25?C in water. Determine the individual column limit according to product instructions for use.
