品牌:美国 NewEast Biosciences
货号: 26039
抗原:Synthetic peptide
规格:100 微升
产品全名:Anti-BRaf(V600E) Mouse Monoclonal Antibody
应用: ELISA, WB, IF, IHC
种属反应性的选择:Human, Mouse and Rat
物种/宿主:Mouse
货号: 26039
基因符号: BRaf
描述: Anti-BRaf(V600E) Mouse Monoclonal Antibody
背景介绍: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers
免疫原: A synthetic peptide from the internal region of BRaf(V600E), human origin
经过测试的应用: ELISA, WB, IHC, IP
推荐稀释比:
ELISA: 1: 100-1: 5000
WB: 1: 100-1: 2000
IHC: 1: 100-1: 1000
浓度: 1 mg/ml
宿主: Mouse
形式: Liquid
克隆性: Monoclonal
亚型: IgG2b
纯度: Purified from ascites
防腐剂: No
成分:PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性: Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates.
储存条件: Store at -20°C. Avoid repeated freezing and thawing
蛋白印迹：
Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody.
Purified His – tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His – tagged B-Raf( V600D ) (lane 3) , His – tagged B-Raf( V600A ) (lane 4) , His – tagged B-Raf( V600G ) (lane 5) , His – tagged B-Raf( V600K ) (lane 6), His – tagged B-Raf( V600R ) (lane 7), and His – tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039) .
免疫荧光：
Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039).
Immunohistochemistry:
B-Raf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.
Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti-BRaf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C. An HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as the secondary antibody. Allele-specific PCR validated to be Negative for BRAF V600E.
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处，也是目前国内经销处。
目前客户使用产品发表超过1000谷歌学术文献，其中论文分布排名前50刊物上。
NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体**的信号转导，包括跨膜受体，例如介导味觉、嗅觉和视觉的那些，（2）核糖体的蛋白质生物合成，（3）调节细胞分化、增殖、分裂和运动，（4）蛋白质通过膜的易位，（5）细胞内囊泡的运输，以及囊泡介导的分泌和摄取，通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
更多产品类目和技术资料可查看：武汉费斯德生物科技有限公司官网。
