品牌:美国 NewEast Biosciences
货号: 26903
抗原:Full Recombinant Active Rac
规格:30 微升
产品全名:Anti-Rac1-GTP Monoclonal Antibody
应用: IP, IHC, IF
种属反应性的选择:Human, Mouse and Rat
物种/宿主:Mouse
货号: 26903
规格: 30 μL
基因符号: RAC1
描述: Anti-Rac1-GTP Monoclonal Antibody
背景介绍: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
免疫原: Recombinant full length protein of active Rac1
经过测试的应用: IP, IHC, IF
推荐稀释比: IP: 1 μg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
浓度: 1 mg/ml
宿主: Mouse
形式: Liquid
克隆性: Monoclonal
亚型: IgM
纯度: Purified from ascites
防腐剂: No
成分: PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性: Anti-Rac1-GTP monoclonal antibody recognizes active Rac1 from vertebrates.
储存条件:Store at -20°C. Avoid repeated freezing and thawing
免疫沉淀/蛋白质印迹:
Rac Activation Assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-Rac-GTP monoclonal antibody (Cat. # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rabbit polyclonal antibody (Cat. # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel).
免疫荧光:
Immunofluorescence for the active Rac using Anti-Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 oC methanol or 4% paraformaldehyde (fixation time 1 hr) and stained with antibody at 1: 1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1: 1000.
